Fig. 4. EBNA1-mediated segregation in KY320.hEBP2. KY320.hEBP2, which contains an integrated hEBP2 gene under control of a constitutively active promoter, was used in plasmid loss assays over 11 generations as described in Figure 2. The assays were conducted in the presence of either wild-type EBNA1 (+) or the EBNAΔ325–376 mutant (Δ325–376), or in the absence of EBNA1 (–).