Table I. Stability of segregation plasmids in the presence and absence of EBNA1 and hEBP2.
| Segregation plasmid | EBNA1 expression | hEBP2 expressiona | % Plasmid stabilityb |
||
|---|---|---|---|---|---|
| KY320 | W303-1A | KY320.hEBP2 | |||
| pRS314 | – | – | 53 | 74 | NA |
| pRS314 | – | + | NA | NA | 68 |
| YRp7FR | + | + | 62 | 80 | 71 |
| YRp7FR | + | – | 2.1 | 0.9 | NA |
| YRp7FR | – | + | 1.4 | 1.5 | 1.8 |
| YRp7 | + | + | 2.4 | 1.3 | 1.2 |
| YRp7FR | Δ325–376 | + | 0.9 | 0.7 | 2.4 |
| YRp7FR | + | 1–220 | 1.5 | 1.2 | NA |
| YRp7 | – | – | 1.3 | 1.1 | NA |
| YRp7 | – | + | NA | NA | 1.9 |
NA, not applicable.
ahEBP2 was expressed from the p425PGK.hEBP2 plasmid in KY320 and W303.A1, and from the integrated gene in KY320.hEBP2.
bPlasmid stabilities were determined after 11 generations as described in Materials and methods. Values shown are averages from two to four experiments for KY320 and W303.A1, and are the results of the single experiment in Figure 4 for KY320.hEBP2.