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Anti–14-3-3 Antiserum Specifically Recognizes Wheat Germ 14-3-3 Proteins.
Soluble proteins from a wheat germ lysate were separated by SDS-PAGE, transferred to nitrocellulose membranes, and incubated with an antiserum against a pea 14-3-3 protein either in the absence (−) or presence (+) of 2 μg of recombinant protein (14-3-3-ex).
