Figure 6.
Precursor-Containing Complex Dissociates in an ATP-Dependent Manner.
(A) Freshly translated 35S-preSSU was fractionated by size exclusion chromatography (see Figure 4). Fractions 11 to 13 were combined, concentrated by ultrafiltration to a final volume of ∼150 μL, and divided into equal aliquots. Each aliquot was incubated for 15 min at 25°C in the absence (−ATP) or presence of different ATP concentrations as indicated. The incubation mixture was centrifuged at 165,000g for 30 min. Proteins present in the supernatant (S) or pellet (I) were separated by SDS-PAGE, transferred onto nitrocellulose, and subjected to autoradiography.
(B) Quantification of three independent results shown in (A). The sum of radioactivity in the soluble and the insoluble fractions, as determined by liquid scintillation counting, was taken as 100%. Values are given in percentages.