Figure 2.

Comparison of Photoreactivation (PR) Effects of A. capillus-veneris CRY Genes with E. coli Photolyase on Survival Rates of the Photolyase-Deficient E. coli Strain.

The photolyase-deficient strain SY2(DE3) carrying each CRY cDNA in the pCAL-n-EK expression vector was exposed by UV light and plated in duplicate. Before overnight incubation in the dark, one plate of duplicates subsequently was irradiated with blue light (+B) for PR, but the others were not (−B). Each symbol represents the average of three independent survival rates of the cells carrying the following plasmids: pCALCRY1, closed circles; pCALCRY2, open circles; pCALCRY3, closed triangles; pCALCRY4, open triangles; pCALCRY5, closed squares; pCALPHR, open squares with positive control; and pCALSTOP, pluses with negative control.