Figure 5.

Effects of Pulse Irradiation with Red, Far-Red, and Blue Light on the Regulation of CRY4 and CRY5 Gene Expression.

The effects of subsequent pulse irradiation (10 min each) with either red light (R) and far-red light (FR) or blue light (B) and far-red light (FR) on CRY4 and CRY5 transcript accumulation were measured using competitive PCR. Four-day dark-imbibed spores were irradiated in turn with red light and far-red light pulses or with blue light and far-red light. After 24- and 12-hr (h) dark incubation (for CRY4 and CRY5, respectively), the expression rates shown were observed. CRY5 was also simultaneously irradiated with continuous blue light (Bc) and far-red light (FRc).

(A) Representative gel images of competitive PCR for CRY4 and CRY5. (B) Relative amounts of CRY4 and CRY5 mRNAs. Each bar shows the average from two experiments. The gray, dotted, and black horizontal lines in each panel indicate the amounts of CRY4 and CRY5 cDNAs after the same lengths (24 and 12 hr) of continuous red (Rc) and blue (Bc) light exposure and of darkness (Dc), respectively (see Figure 4B).