Figure 2.

Attenuation of ClpP by the clpP-AUU Mutation.

(A) Immunoblot of total cell proteins taken from exponential-phase TAP cultures of the wild-type (WT), BI, clpP-AUU, and clpP-AUU2 (an independent transformant carrying the clpP-AUU mutation) strains. (B) Temperature-dependent attenuation of ClpP. Samples were taken from cultures of the wild type (WT) or clpP-AUU in exponential phase and grown at 18, 25, or 32°C. The amount of LMM-ClpP and HMM-ClpP was determined by immunoblotting with a ClpP antibody and PhosphorImager analysis. The value 100 represents the total immunoreactivity in the wild type at 18°C. AU, arbitrary units.