Figure 5.

Analysis of petC-ac21 clpP-AUU Double Mutants.

(A) Immunoblot of total cell proteins from parental strains ac21 and clpP-AUU, and two double mutant progeny from the cross, harvested during exponential phase. (B) Stability of cytochrome f, subunit IV, and the Rieske protein in the ac21 (X's) and petC-ac21 clpP-AUU mutant (open circles) strains. Chloramphenicol was added at time 0 in early exponential- phase cultures. Immunoreactivity is plotted as the percentage of the initial level in each culture. Cyt f, cytochrome f; Su IV, subunit IV.