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. 2003 Dec 23;1:e012. doi: 10.1621/nrs.01012

Figure 3. Primer set validation.

Figure 3

(a) Example of a valid primer set for mouse LXR- α using SYBR® Green. The presence of a single peak in the dissociation curve and the -3.3 slope and 0.99 R2 value of the standard curve plot are indicative of a good set of primers. (b) Example of an invalid primer set for mouse HSD due to the amplification of non-specific products, as indicated by the presence of multiple peaks in the dissociation curve. This may be an effect of non-specific priming or primer dimerization. Note that the slope of the standard curve plot (-3.2) is within the acceptable range of a valid primer set, but the dissociation curve renders this set of primers unacceptable. (c) Example of an invalid primer set for mouse PNR due to poor amplification. Note the unacceptable slope (-2.28) in the standard curve plot, and the presence of multiple peaks in the dissociation curve.