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. 2003 Jan;14(1):14–25. doi: 10.1091/mbc.E02-06-0335

Figure 7.

Figure 7

GFP-RIII and GFP-WD40 repeat domains partially fractionate with the Triton-insoluble cytoskeletal pellet. Triton-soluble and Triton-insoluble samples of DH-1 GFP, DH-1 GFP-RIII, and GFP-WD40 repeat transformants were prepared and run on duplicate SDS-PAGE gels. One gel was stained with Coomassie blue, and the other was subjected to Western blot analysis probed with an anti-GFP antibody. Lanes: 1, DH-1 GFP Triton-soluble sample; 2, DH-1 GFP Triton-insoluble sample; 3, DH-1 GFP-RIII Triton-soluble sample; 4, DH-1 GFP-RIII Triton-insoluble sample; 5, DH-1 GFP-WD40 repeat Triton-soluble sample; 6, DH-1 GFP-WD40 Triton-insoluble sample.