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Bni4V-A/F-A fails to localize properly to the bud neck. (A) Indirect immunofluorescence of Bni4 in a bni4Δ1::TRP1 strain (YLK78), a wild-type strain (YLK74), and in a bni4V-A/F-A strain (YLK76), as described in Materials and Methods. Bni4 was visualized using anti-Bni4 antibody (DeMarini et al., 1997). Bar, 5 μm. (B) Immunoblot analysis of the strains described in A. (C) GFP fluorescence in bni4V-A/F-A:GFP (YLK84) and BNI4:GFP (YLK80) strains.
