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Levels of Bni4-YFP at the bud neck are reduced in glc7-129 cells. (A) Two-hybrid analysis of Bni4 and Glc7. Strain PJ69-4A was cotransformed with pAR5, encoding Bni4, and either pHH149 or pKT1703, which encode Glc7 and glc7-129, respectively. (B) Bni4-YFP was visualized in either KT2153 (GLC7) or in KT2155 (glc7-129) cells. Bar, 5 μm. (C) Fluorescence at the bud neck in the small- and medium-budded cells of GLC7 or glc7-129 strains from B was measured (p < 0.001).
