Figure 1.

FADD-DD selectively kills normal prostate epithelial cells. Normal primary prostate epithelial cells or DU145 tumor cells were infected with Dox-regulated adenoviruses expressing YFP (a,b,c,d,i,j,k,l) or YFP-tagged FADD-DD (e,f,g,h,m,n,o,p) and a TetR virus. Adjacent panels show phase and fluorescence images of the same field. Expression was induced by removing Dox. FADD-DD caused normal cells to die (e and f) but did not affect DU145 cells (m and n). YFP had no effect in either cell type. The lower panel shows total protein probed with anti-YFP. Equal amounts of protein were expressed in each case. The top band is a cross-reacting protein serving as a loading control.