Figure 2.

FADD-DD inhibits Fas-induced apoptosis in prostate tumor cells. DU145 cells were infected with the YFP or YFP-FADD-DD adenoviruses, expression was induced by removing Dox, and then cells were treated with an anti-Fas agonistic antibody. (A) Cellular morphology indicating Fas-induced apoptosis that is inhibited by YFP-FADD-DD but not by YFP. (B) Total protein samples Western blotted with anticaspase 8. Fas stimulates caspase 8 as indicated by the appearance of the processed caspase 8 band that is inhibited by YFP-FADD-DD.