Figure 3.

Caspase activation by FADD-DD. Normal prostate epithelial cells were infected with YFP or YFP-FADD-DD adenoviruses or treated with 300 mM sorbitol as a control. Cells were harvested and total protein extracts separated on gels and Western blotted with the indicated antibodies. Panel A shows that FADD-DD causes the appearance of cleaved forms of cytokeratin 18 and PARP that are blocked by treatment with zVAD.fmk. Panel B shows that active forms of caspase 9, 7, 3, and 6 but not caspase 8 are induced by FADD-DD.