Figure 4.

Caspases and serine proteases contribute to FADD-DD–induced apoptosis. Normal prostate cells were injected with the YFP control of YFP-FADD-DD expression plasmids as indicated then treated with the caspase inhibitor (zVAD.fmk, 0.1 mM) or serine protease inhibitor (AEBSF, 0.3 mM) as indicated. The percentage of injected cells that survive were determined after 24-h incubation, indicating that neither zVAD.fmk or AEBSF alone could prevent FADD-DD–induced cell death but the combination of both inhibitors did prevent death. Data shown are means ± SEM from five separate experiments.