Figure 5.

Caspases and serine proteases regulate different aspects of FADD-DD–induced apoptosis. Normal prostate cells were injected with the YFP-FADD-DD expression plasmid then incubated with zVAD.fmk or AEBSF as indicated. Time-lapse fluorescence microscopy was performed to monitor the response of each injected cell. The images show overlayed fluorescence and phase images of the same cells at the beginning and end of the experiment indicating that FADD-DD causes membrane blebbing and cell fragmentation that is blocked by zVAD.fmk but not by AEBSF. Quicktime movies of these experiments are contained in the supplementary material.