Figure 1.

Synthetic gene encoding for DI of human β₂GPI. The synthetic gene encodes for DI (underlined amino-acids) and an OmpA leader sequence. Codons were chosen for optimal expression of DI in E. coli by Juniper, altering 67% of the native sequence (boxed codons). Synthetic oligonucleotide primers are denoted by grey shading; oligonucleotides of the upper DNA sequence are read 5' to 3' (left to right) and the oligonucleotides of the lower DNA sequence are read 3' to 5' (left to right). Restriction sites are denoted in light grey font.