Figure 3.

Greater expression of DI seen with gene optimised for E. coli expression. Western blot analyses of recombinant his₆-tagged DI. Protein was transferred onto a nitrocellulose membrane from a 15%-SDS-PAGE gel and probed with an anti-his₅ antibody. Lane 1, his₆-tagged molecular weight protein markers; lane 2, DI expressed using the native cDNA human sequence; lane 3, DI expressed using sequence optimised for E. coli. Equal volumes of periplasmic extract were applied to lanes 1 and 2 on the protein gel.