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. 2003 Jan;14(1):78–92. doi: 10.1091/mbc.E02-04-0182

Figure 5.

Figure 5

Overexpression of survivinM34DsRed causes defective cytokinesis, apoptosis, and impaired cellular growth. (A) Multinuclearity of HeLa cells caused by high-level expression of the survivinM34DsRed mutant. a and b, multiple nuclei and aberrant cleavage of cell bodies. C, triple merge of a cell provirally coexpressing mutant survivinM34DsRed and α-tubulin and being stained with TO-PRO3. DNA-staining (blue), α-tubulin (green), and merge of survivinM34DsRed and DNA (pink). Note the irregular appearance of the microtubular cytoskeleton and cleavage of membranes containing DNA or DNA-bound survivinM34DsRed. Bar, 20 μm. (B) Incidence of multinuclearity in adherent HeLa cells transduced with 50 MOI of particles coding for survivinDsRed, survivinM34DsRed, or as control, DsRed. Of each cell line 2500 cells were analyzed. Bars, ±SD. p < 0.05 for HeLa survivinM34DsRed cells compared with HeLa DsRed cells and HeLa survivinDsRed cells, respectively. (C) TUNEL analysis of HeLa cells with high-level expression of DsRed, survivinDsRed, and survivinM34DsRed. Of each cell line 2000 cells were analyzed. Bars, ±SD. *p < 0.05 for HeLa survivinM34DsRed compared with HeLa DsRed and HeLa survivinDsRed, respectively. (D) Impaired cellular growth of HeLa cells overexpressing survivinM34DsRed. Cells (105) were plated per well in culture dishes. At the indicated time points cells were trypsinized and counted. Data represent means of five independent experiments. Bars, ±SD. *p < 0.05 or **p < 0.01 when cells overexpressing survivinM34DsRed were compared with the DsRed-transduced control cell line.