Figure 6.
High-level expression of p34cdc2 phosphorylation-defective survivin causes aberrant cleavage of HeLa cells. (A) Time-lapse video imaging of a HeLa cell line with high-level expression of survivinDsRed fusion protein. Images were acquired at intervals of 30 s. Start of chromosomal segregation was set as time zero. (B) HeLa cell line with high level expression of mutant survivinM34DsRed. Note that not all survivinM34DsRed signals accumulated at the cleavage furrow. Arrowheads indicate localization of signals in the cytoplasm of the daughter cells. Arrows depict blebbing of the daughter cells in the vicinity of the premature midbody at telophase. (C) Differential interference contrast microscopy of HeLa cells with high expression of survivinDsRed (a, c, and e) or mutant survivinM34DsRed (b, d, and f). Arrows indicate membrane blebbing at the cleavage furrow at telophase. Arrowheads depict cleavage furrows. Bars (A and B), 15 μm; and C, 20 μm.