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. 2006 Feb 16;3:14. doi: 10.1186/1742-4690-3-14

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Copyright © 2006 Turk et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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TAR structure of BF recombinant variants. TAR secondary structure of CRF12_BF (ARMA159), and BF recombinant isolates F27, F55 and F92 are shown. B (NL4-3) and F (isolate 93BR020) subtype TARs were used as reference. Nucleotide changes are highlighted. Changes were found at positions 31 (U31), 32 (G32) and 33 (G33) in these isolates. Nucleotide position 32 is one of the essentials for CycT1-Tat interactions with TAR RNA. Nucleotides U31 and G33 are not critical, but they contribute to the stabilization of the RNA-protein complex.