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. 2006 Feb 16;3:14. doi: 10.1186/1742-4690-3-14

Table 1.

List of primers used for PCR amplification

Target region Primer name (sequence 5'-3')
LTR 1R F: LTR1 (cacacaaggctayttcctga)
R: JL17 (cattctgcagcttcctcattgat)
2R F: LTR3 (tggatggtgctwcaagytagt)
R: LTRrev (tgctagagattttccacactgac)
tat 1st exon 1R F: polseq2 (cgggtttattacagggacagc)
R: ES33 (cattgccactgtcttctgctc)
2R F: TECORI (cagaataggaattctgcgacagagaag) *1
R: TE1 (gggatatgggttgctttgatagagaagc)
tat 2nd exon 1R F: KS2mod (ttctatagtggatagagttaggaaggg)
R: OFM19mod (gcactcaaggcaagctttattgaggc)
2R F: TE2 (gcttctctatcaaagcaacccatatccc)
R: TXHOI (acaggctcctcgaggtcgtccc) *2
β-actin F: β1 (ggacctgactgactacctcatgaa)
R: β2 (gatccacatctgctggaaggtgg)

1R = 1st round, 2R = 2nd round. Introduced EcoRI (*1) and XhoI (*2) restriction sites are underlined. F: Forward. R: Reverse.