Figure 3.

Movement of intravacuolar nuclear envelope vesicles. Images taken at 90-s intervals of an Nvj1-EYFP–expressing wt cell containing a number of blebs and vesicles. At least two Nvj1p-EYFP/FM4-64–stained vesicles moved freely in the vacuole (white arrowheads). An immobile Nvj1p-EYFP/FM4-64–stained bleb/vesicle located away from the NV junction but adjacent the vacuole membrane (pink arrowhead). In addition to blebs associated with the NV junction, this cell contains a mobile vesicle stained only with FM4-64 (purple arrowhead). Cells were grown in YPD, stained with FM4-64, starved for 3 h in SD-N, and stained with Hoechst as described in MATERIALS AND METHODS.