Figure 5.

Degradation of nuclear envelope blebs is PEP4-dependent and APG7-independent. (A) Frequencies of Nvj1p-EYFP/FM4-64–stained nuclear envelope blebs and vesicles in wt (white bars), apg7-Δ (gray bars) and pep4-Δ (black bars) BY4741 cells. Each bar represents the mean of three independent counts of at least 100 cells. (B) Nvj1p-EYFP–labeled blebs and/or vesicles in wt, apg7-Δ, and pep4-Δ cells (arrowheads). (a–c) Cells stained with Nvj1p-EYFP (green). (d–f) Overlays of a, b, and c with Hoechst-stained chromatin (blue), FM4-64 for the vacuole membrane (red), and differential interference contrast. P_CUP1-NVJ1::EYFP expression in the indicated strains was induced for 1 h with 0.1 mM Cu²⁺, shifted to SD-N medium for 3 h, and stained with Hoechst as described in MATERIALS AND METHODS.