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Effect of brefeldin A. (A) Images of GFP-CI-MPR–expressing cells were taken at 2-s time intervals during BFA treatment. Inverted images at the indicated time intervals (min:sec) are presented. The first frame (0:00) shows the cell immediately after adding BFA. Bar, 10 μm. (B) Growth of tubular elements in the absence (BFA−) or the presence (BFA+) of brefeldin A. Four examples of growing TGN tubules are shown.
