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The effect of OPN on the bone resorption of WT and OPN−/− osteoclasts. Confocal microscopy analysis of the resorption pits on dentine slices. (A, B, E, and F) Wild-type osteoclasts; (C, D, G, and H) OPN-deficient osteoclasts. (A, C, E, and G) Osteoclasts treated with PBS (control); (B, D, F, and H) osteoclasts treated with OPN. (A–D) Lower power view of resorption pits using 10× objective. (E–H) Higher power magnification of resorption pits using 60× objective. Bar, 25 μm.
