Figure 9.

The effects of neutralizing antibodies to OPN, β₃, or CD44 on OPN- induced bone resorption. The effects of antibodies to OPN, β₃, or CD44 were assessed in OPN-induced bone resorption activity of osteoclasts isolated from WT (A–F) and OPN−/− (G–L) mice. OPN-induced bone resorption was reduced when cotreated with antibodies to β₃ (D and J), CD44 (E and K), or OPN (C and I) in both WT and OPN−/− osteoclasts. Osteoclasts treated with both nonimmune IgG and OPN (F and M) had similar effect as that of OPN-treatment (B and H). Pits were viewed under 40× objective in a phase contrast microscope and photographed. About three slices were used for each treatment. The experiment was repeated three times. The results represent one of the three experiments performed.