Overexpression of p47 or Ufd1p does not induce cell vacuolation. The C-terminally FLAG-tagged full-length SVIP (a and f), untagged SVIP (b and g), SVIP fused to the N-terminal GST (c and h), N-terminally FLAG-tagged p47 (d and i), or N-terminally FLAG-tagged Ufd1p (e and j) was expressed in HeLa cells. The cells were double stained with the antibodies against BiP (a–e), and FLAG (f, i, and j), SVIP (g), or GST (h).