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Sam68 accumulates in the cytoplasm of PRMT1 −/− ES cells. Wild-type ES cells (PRMT1 +/+; A and B) or ES cells homozygotes (PRMT1 −/−; C and D) for a null mutation in the prmt1 gene were fixed and immunostained using the anti-Sam68 AD1 antibody followed by a secondary antibody conjugated to Alexa 488. Cells were then visualized using a confocal microscope (Carl Zeiss).
