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. 2003 Jan;14(1):302–312. doi: 10.1091/mbc.E02-05-0247

Figure 4.

Figure 4

Requirement for GSK-3 in STRE-mediated transcription. (A) Stress-induced transcription in the gsk-3 null mutant. After cells of strains W303a stre (wild-type) and YTA003W stre (Δgsk-3) were exposed to heat shock or salt stress for 1 h, β-galactosidase activities of the cells were assayed and expressed in Miller units (Miller, 1972). (B) Effects of Mck1p on stress-induced transcription in the gsk-3 null mutant. Cells of strains W303a stre and YTA003W stre transformed with the indicated plasmids were exposed to heat or salt stress for 1 h. (C) Effects of Mck1p on stress-induced transcription in the Δmck1 Δmds1 msn2 msn4 quadruple mutant. Cells of strains W303a stre (wild-type), YTA002W stre (Δmck1 Δmds1 double mutant), and YYH001 stre (Δmck1 Δmds1 msn2 msn4) transformed with the indicated plasmids were exposed to heat or salt stress for 1 h. The results shown are means ± SEM of three independent experiments. Note that the experiments shown in A used YPD medium, whereas those shown in B and C used synthetic media. The differences in the absolute values of transcription observed may reflect this difference in medium.