Figure 2.

Submitochondrial localization of the COX3 mRNA translational activator proteins Pet54p, Pet122p, and Pet494p. Mitochondria were isolated from yeast a strain expressing HA-tagged form of Pet122p-HA and Pet494p-HA (CAB30). Purified mitochondria (MT) and mitoplasts (MP) were subjected to digestion with proteinase K (100 μg/ml) in presence (+) and in absence (−) of 1% octyl-glucoside, a nonionic detergent. Treated samples were analyzed by Western blotting by using anti-HA-HRP to detect Pet122p-HA and Pet494p-HA. Filters were stripped and reprobed with antisera against Pet54p, the soluble intermembrane space protein cytochrome b₂ (Cyt b₂) and the soluble matrix marker α-ketoglutarate dehydrogenase (αKDH).