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Colocalization of VEGFR-2 and numerous regulatory proteins in caveolae membranes isolated from endothelial cells. Caveolae membranes were isolated from ECV304 cells (A and B) or BAEC (C and D), after ultracentrifugation of plasma membranes on Optiprep gradients (Smart et al., 1995). Equivalent amounts of proteins from the lysates and purified membranes (5 μg) were separated by SDS-PAGE and were immunoblotted with the indicated antibodies.
