Table 1.
Detection of Experimental Rhinovirus 16 Aerosols by Semi-nested RT-PCR of Filter Samples
Nebulizing Solution | ||||||
Run Number | TCID50 per ml | Total ml Nebulized | Fractional Sampling Time per Filtera | Yieldb | Predicted TCID50 per Filterc | Positive Filters/Total |
VA Run 12 | 16.1 | 15.2 | 0.136 | 0.041 | 1.4 | 3/3 |
VA Run 17 | 10.1 | 24.2 | 0.085 | 0.103 | 2.1 | 3/3 |
VA Run 13 | 6.5 | 15.0 | 0.142 | 0.093 | 1.3 | 2/3 |
VA Run 14 | 3.3 | 16.7 | 0.119 | 0.072 | 0.5 | 0/3 |
aSampling time of each filter (10 minutes) divided by total run time. bFluorescence yield (observed/expected) for sampling run. cPredicted = TCID50 per ml * Total ml nebulized * Fractional sampling time per filter * Yield.