FIGURE 8.

Back-to-back dimerization of SecYEβ. (A) Dimer of SecYEβ viewed from the periplasm. Each monomer is shown in the same representation as in Fig. 1 with the exception of the color scheme of the left monomer (blue for SecY, yellow for SecE, and red for Secβ). The plugs in the dimer are without well-defined helical structure and are shown in green. (B) Destabilization of the plugs. The RMSD of the plugs (residues Ile⁵⁵ to Gly⁶⁵) is shown after fitting the entire SecY to the crystal structure at each point. Presented in black is the RMSD for the plug of the monomer during sim0 (see Table 1); shown in red and blue are the RMSD values for each plug from the dimer as evaluated during simulation sim5.