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. 2006 Jan 20;90(7):2548–2562. doi: 10.1529/biophysj.105.069815

FIGURE 2.

FIGURE 2

Autocorrelation curves of eGFP fluorescence signals and their corresponding fits (solid lines), the diffusion times of which are given in the figure. Panel a corresponds to the data of the vesicle plotted in Fig. 1 b, before correction of the fluorescence decay (dotted line) and after correction (dashed line). Panel b shows a comparison between the autocorrelation obtained in solution (dashed line) and in a cell, after correction (dotted line). The amplitude of the autocorrelation function of eGFP in cell has been multiplied by a factor 20 for convenience; in addition, a slow component (τD = 1566 μs) had to be added to perform a satisfactory fit, up to very long times. In all cases the laser power is 13 μW.