TABLE 1.

Diffusion constants

	Literature	Solution	Vesicles	Cells
FITC	—	280 ± 5 μm2/s	280 ± 5 μm2/s	—
Fluorescein	—	280 ± 5 μm2/s	280 ± 5 μm2/s	—
Strep-fluo.	60 μm2/s*	¶	73 ± 15 μm2/s	—
	74 μm2/s*
FD4	173 μm2/s†‖	143 ± 7 μm2/s	173 ± 20 μm2/s	26 ± 4 μm2/s
FD70	40,2 μm2/s†	33 ± 3 μm2/s	47 ± 10 μm2/s	—
FD250S	19 μm2/s†	24 ± 5 μm2/s	26 ± 2 μm2/s	—
eGFP	78 μm2/s in sol.‡	70 ± 7 μm2/s	90 ± 10 μm2/s	21 ± 4 μm2/s
	25 μm2/s in cyto.‡
	33 μm2/s in nuc.§

Mean values and SD of the diffusion constants of the molecules used in this work are defined below.

^*(34).

^†Calculated from the molecular weight (35).

^‡(36).

^§(37).

^¶Aggregation and /or important triplet population prevent a reliable determination of the diffusion constant.

^‖Small dextran molecules (MW = 70 × 10³) have been found to be approximately seven times slower in nucleus than in water solution (37).