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. Author manuscript; available in PMC: 2006 Mar 17.
Published in final edited form as: Antisense Nucleic Acid Drug Dev. 2003 Feb;13(1):45–55. doi: 10.1089/108729003764097331

Figure 2.

Figure 2.

Agarose gel analysis of PCR products derived from the random oligonucleotide priming and amplification using the RT-ROL protocol on HIV-2 leader region RNA. Lane L: DNA ladder (New England Biolabs, 100bp); Lane 1: negative control reaction containing no random oligonucleotide during the reverse transcription step; Lane 2: positive control reaction in which oligonucleotides complementary to the PBS and 145 regions were added to the reverse transcription reaction instead of the random oligonucleotides; Lane 3: complete RT-ROL reaction showing several amplification products arising from binding of random oligonucleotides and subsequent reverse transcription and PCR amplification.