Figure 5.

Antisense oligonucleotide binding to 1-381 RNA under native conditions. A) Ethidium bromide stained gel showing the effects of binding substoichiometric radiolabeled oligonucleotide (lanes 2, 4, 6, 8, 10, 12) or a 20:1 excess of unlabeled oligonucleotide (lanes 3, 5, 7, 9, 11, 13) to viral RNA under dimerization conditions and native electrophoresis (see Materials and Methods). Oligonucleotides used are indicated below lanes. Note that some oligonucleotides stained more or less efficiently with ethidium bromide (e.g. oligonucleotide 172 appears as a fainter band). However, oligonucleotide concentrations were accurately determined by spectrophotometric measurements. B) Autoradiogram of the same gel as in panel A showing the partitioning of the radiolabeled oligonucleotides with respect to the viral RNA. An asterisk (*) below the lane indicates presence of radiolabeled oligonucleotide, a plus sign (+) indicates the presence of a 20-fold excess of unlabeled oligonucleotide, and a minus sign (-) indicates that no oligonucleotide was added.