TABLE 2.
Oligonucleotides used in this study
| Primer or probe name | Target gene | Oligonucleotide sequence (5′ → 3′)a | Amplicon size (bp) |
|---|---|---|---|
| Primers | |||
| 1Slt224 | stx1 | ATG TCA GAG GGA TAG ATC CA | 185 |
| 1Slt385 | stx1 | TAT AGC TAC TGT CAC CAG ACA AT | |
| 2Slt537 | stx2 | AGT TCT GCG TTT TGT CAC TGT C | 160 |
| 2Slt678b | stx2 | CGG AAG CAC ATT GCT GAT T | |
| Probes | |||
| 1SltB1FAM | stx1 | GCG AGG CGC TTT GCT GAT TTT TCA CAT GTT ACC CCT CGC | |
| 2SltB1TET | stx2 | GCG AGG CAC TGT CTG AAA CTG CTC CTG TCC TCG C |
a
The underlined sequences of each probe constitute the stem of each molecular beacon. FAM, 6-carboxyfluorescein; TET, tetrachloro-6-carboxyfluorescein.