FIG. 1.

Multiplex RT-PCR typing of five representative Malawian neonatal rotavirus strains. RT-PCR type-specific products were resolved on a 2% agarose gel and stained with ethidium bromide. Lane 1, 123-bp DNA ladder (GIBCO-BRL, Long Island, N.Y.). Molecular weights are indicated to the left. Lanes 2 to 7, G typing. Second-amplification RT-PCR products of known serotype G1 control strain (lane 2) and Malawian neonatal strains (serotype G8, lanes 3 to 7) are shown. Lanes 8 to 13, P-typing. Second-amplification RT-PCR products of known genotype P[8] control strain (lane 8) and Malawian neonatal strains (genotype P[6], lanes 9 to 13) are shown. The first-amplification con2/con3 products are indicated (arrow).