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. 2002 Apr;40(4):1333–1338. doi: 10.1128/JCM.40.4.1333-1338.2002

TABLE 2.

Primers used in the sequencing of the entire rpoB gene in S. lugdunensis, S. intermedius, S. saccharolyticus, and S. caprae

Primer name Primer sequence (5′-3′) Positiona Tm (°C)
30F GGTTTAGGATTAAAAGATGC 30-50 41
192F GAAGAAGTTGGAGCTACTG 192-211 44
806F AATAAGAGCAGGGAAAGAAAC 806-827 43
920F AAAGAAAAGAATGAATGAACTT 920-942 39
1165F TATGCTTATGGTATTTAGCTA 1165-1186 39
1302F AAACTTAATAGAAATTCAAACTAAA 1302-1327 58
1450F GTTCAAACGATAAATAGAGAA 1450-1471 39
1741F GAAACAGATGCTAAAGATGT 1741-1761 41
1850F CCATATACTGCGAGTGGGAA 1850-1870 47
2245F TAGAAATTCAATCAATTAAGTATATG 2245-2271 62
2309F TTGGTAATGCTTTACCAGAT 2309-2329 41
2334F TGCATTACACCAGCAGATATCATTG 2334-2359 70
2412F GATGATATTGACCATTTAGG 2412-243 41
2534F TGAAAGAATGTCAATTCAAGA 2534-2555 39
2663F AAACCCATTAGCTGAGTT 2663-268 38
2995F TGGTCGTTTCATGGATGATGAAGTTG 2995-3119 74
2924F AAGATAGCTATGTTGTAGCA 2924-2944 41
3200F CTTAGAGAACGATGACTCTAA 3200-3221 43
3498F TAGTTGGTTTCATGACTTGGGA 3498-3520 46
3550F TTGAAAGTCCAACAAAGCAA 3550-3570 38
3843F GGTAAAGTAACGCCTAAAGGT 3843-3864 45
4494F TGGAGGTATGGGCACTTGAA 4494-4514 47
1759R ACATCTTTMGCATCTGTTTC 1779-1759 48
1460R ATCGTTTGAACGCCACTCTT 1480-1460 45
1910R TCATAGTAAGTTTGCGCCAT 1930-1910 43
2309R ATCTGGTAAAGCATTACCAA 2329-2309 41
2334R CAATGATATCTGCTGGTGTAATGCA 2354-2334 68
2432R CCTAAATGGTCAATATCATC 2452-2432 41
2573R CGAATATTAATTAATTGTTG 2593-2573 34
2892R GTGATAGCATGTGTATCTAAATCA 2912-2892 64
2915R TAACTATCTTCTTCATCAGC 2935-2915 41
2924R TGCTACAACATAGCTATCTT 2944-2924 41
2995R CAACTTCATCATCCATGAAACGACCA 3015-2995 74
Cm32b ATGCAACGTCAGGCCGTTCCG 3211-3191 64
3321R AGACGACGAACAGAATTTCA 3341-3321 56
3610R GCTCGAATGATAACGTGATT 3630-3610 43
4139R ACTTGTCCrATGTTCATACG 4159-4139 44
4502R CATATGCTTCAAGTGCCCATA 4523-4502 45
4508R CCAAGTGGTTGTTGTGTAAC 2428-4508 45
4871R TTTAGAGCTTTCACTGTTTG 4891-4871 41
5000R CACCATATGACCAAGAACGAA 5021-5000 45
5018R CAATCAAGGAGCCTACCTCCTT 5040-5018 50
5030R GAAATTATTTACATCAATCAA 5051-5030 36
5041R TAACTATCTTCTTCATCAGC 5061-5041 41
5085R CCCAGTCTTTTGTAGGTCCG 5105-5085 49
5188R CCCATTCTTTCACGACGTAC 5208-5188 47
a

Position relative to the S. aureus rpoB sequence.