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Reactivity of HUS patient sera with TagA-producing E. coli JM109. Lysates of E. coli JM109 carrying either pBluescript (lane 1) or pJCP574 (lane 2) were separated by SDS-PAGE, electroblotted, and probed with convalescent-phase sera from patients with HUS caused by STEC strains belonging to serotype O157:H− (A and B), O111:H− (C, D, and E), or O113:H21 (F), or with sera from healthy controls (G and H). The expected mobilities of TagA and various protein size markers are indicated.
