TABLE 1.
Sample | Tissue type | No. of tachyzoites/ mg of tissuea | Results of:
|
|
---|---|---|---|---|
HPb | Nested PCR | |||
Fetus | ||||
1 | Frozen | 0 | − | − |
Paraffin embedded | 10.2 | + | ||
2 | Frozen | 7.5 | − | + |
3 | Frozen | 3.7 | − | + |
4 | Frozen | 2.9 | − | + |
5 | Frozen | 0 | − | − |
6 | Frozen | 0 | − | − |
7 | Frozen | 13 | + | + |
8 | Frozen | 17.4 | + | + |
Paraffin embedded | 0 | − | ||
9 | Frozen | 26.6 | + | + |
10 | Frozen | 13.2 | + | + |
11 | Frozen | 9.7 | + | + |
Paraffin embedded | 0 | − | ||
12 | Frozen | 0 | + | + |
Mousec | ||||
1 | Frozen | 3.1 | − | + |
2 | Frozen | 0 | − | − |
Paraffin embedded | 0 | − | ||
3 | Frozen | 0 | − | − |
Paraffin embedded | 0 | − | ||
4 | Frozen | 3 | + | + |
Paraffin embedded | 0 | − | ||
5 | Frozen | 109.6d | + | + |
6 | Frozen | 3.5 | + | + |
Controls | Frozen | 0 | − | − |
Values are the means of two or more assays.
HP, histopathological study; a “+” indicates that brain samples showed compatible or characteristic lesions of neosporosis.
Mice 1 and 4 were infected with 5 × 106 Nc-1 tachyzoites subcutaneously, mice 2 and 6 were infected with 106 tachyzoites intraperitoneally, and mice 3 and 5 were infected with 106 tachyzoites subcutaneously.
Parasite tissue cysts were observed in the brain during the histopathologic examination.