TABLE 2.
Repeatability of Neospora Nc5 and 28S rRNA real-time PCR tests in samples of brain from bovine aborted foetuses and inoculated mice
| Sample | No. of tachyzoitesa
|
Amt of brain tissue (mg)a
|
||||
|---|---|---|---|---|---|---|
| Mean | SD | % CV | Mean | SD | % CV | |
| Fetus | ||||||
| 1 | 0 | 0 | 0 | 21.9 | 0.8 | 3.5 |
| 2 | 13.3 | 0.6 | 4.3 | 1.7 | 0.1 | 5.4 |
| 3 | 16.7 | 3.1 | 18.3 | 4.6 | 0.8 | 17.6 |
| 4 | 43.3 | 12.7 | 29.2 | 14.9 | 0.8 | 5.1 |
| 7 | 36 | 1.0 | 2.8 | 2.8 | 0.2 | 8.3 |
| 10 | 46 | 8.5 | 18.6 | 3.5 | 0.4 | 13.0 |
| Mouse | ||||||
| 1 | 22.7 | 4.2 | 18.4 | 7.4 | 1.2 | 16.7 |
| 4 | 19.7 | 7.2 | 36.8 | 6.6 | 1.5 | 22.9 |
| 5 | 265.3 | 18.1 | 6.8 | 2.4 | 0.2 | 10.5 |
a
The given values are the means of the duplicates of the three different PCR runs.