Table 1.

NSMC and NSCS test results for TP53 somatic mutation spectra (H0: mutational bias; H1: selectional bias)

	Sites	Mutations	Hotspots	P(H1)/χ2
Bladder
Synonymous	61	80	12
Non-synonymous	240	775	110	0.107/13.8
Nonsense	36	85	26	0.038
Brain
Synonymous	20	23	2
Non-synonymous	199	812	98	0.998/11.2
Nonsense	14	32	5	0.015
Breast
Synonymous	77	108	19
Non-synonymous	294	1423	171	0.111/27.3
Nonsense	49	73	27	0.052
Liver
Synonymous	34	43	5
Non-synonymous	199	727	99	0.980/14.4
Nonsense	29	50	16	0.040
Lung
Synonymous	56	88	17
Non-synonymous	314	1599	191	0.959/17.9
Nonsense	55	170	31	0.042
Pancreas
Synonymous	10	12	1
Non-synonymous	106	215	30	0.049/1.56
Nonsense	10	17	6	0.058
Ovary
Synonymous	20	21	1
Non-synonymous	192	770	99	0.995/15.7
Nonsense	30	71	12	0.012
Prostate
Synonymous	28	38	7
Non-synonymous	114	195	38	0.926/0.72
Nonsense	9	12	2	0.008
Colon
Synonymous	23	28	4
Non-synonymous	173	592	69	1.000/4.4
Nonsense	19	60	5	0.016
Colorectal
Synonymous	25	28	4
Non-synonymous	203	1097	121	0.000/20.3
Nonsense	29	103	12	0.953
Esophagus
Synonymous	25	32	7
Non-synonymous	215	1064	117	0.025/6.26
Nonsense	50	150	27	0.032
Hematopoietic
Synonymous	26	29	3
Non-synonymous	202	723	99	0.037/13.08
Nonsense	20	37	5	0.018
Larynx
Synonymous	8	10	2
Non-synonymous	108	234	42	1.000/0.60
Nonsense	18	24	5	0.007
Mouth
Synonymous	28	41	9
Non-synonymous	176	413	79	0.988/1.59
Nonsense	33	53	10	0.901
Skin
Synonymous	64	136	12
Non-synonymous	248	563	99	0.931/9.94
Nonsense	25	69	12	0.025
Stomach
Synonymous	44	64	15
Non-synonymous	195	617	88	0.115/1.78
Nonsense	21	52	8	0.029

In the last column, the P-value for the NSMC test (numerator) and the χ² value for the NSCS test (denominator) are shown along with the NSMC test P-value for the hotspots in nonsense sites. The statistically significant values (rejection of H0) are shown in bold type.