Table 2.
Primers pairs and PCR conditions used for the amplification of grapevine flavonoid hydroxylase genes.
| Code | Sense primer | Anti-sense primer | Expected size (bp) | Annealing temp. (°C) |
| F3'H | F3'H_S | F3'H_AS | 825* | 60 |
| F3'5'H | F3'5'H_S | F3'5'H_AS | 1020* | 56 |
| F3 | VvF3'H_S | VvF3'H_AS | 196 | 58 |
| F3-1 | VvF3'H-indel_S | VvF3'H_AS | 166 | 58 |
| F35-1 | Vv3'5'H-1A_S | VvF3'5'H-1A_AS | 156 | 58 |
| F35-2 | Vv3'5'H-2A_S | Vv3'5'H-2A_AS | 152 | 65 |
| F3int | VvF3'H-intron_S | VvF3'H-intron_AS | 255 | 58 |
| F35-1int | Vv3'5'H-1A-intron_S | Vv3'5'H-intron_AS | 589 | 58 |
| F35-2int | Vv3'5'H-2A-intron_S | Vv3'5'H-intron_AS | 593 | 58 |
| 38C24-FM | 38C24-FM_S | 38C24-FM_AS | 312 | 58 |
| 38C24-RM | 38C24-RM_S | 38C24-RM_AS | 330 | 58 |
| 17K14-FM | 17K4-FM_S | 17K4-FM_AS | 327 | 58 |
| 9C17-FM | 9C17-FM_S | 9C17-FM_AS | 358 | 58 |
| 40A22-FM | 40A22-FM_S | 40A22-FM_AS | 327 | 58 |
| 5A23-FM | 5A23-FM_S | 5A23-FM_AS | 218 | 54 |
* in absence of introns.