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. 2006 Jan 30;4:1. doi: 10.1186/1479-0556-4-1

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Copyright © 2006 Coelho-Castelo et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of the pcDNA3-Hsp65 genome integration. Samples of liver tissue from mice immunized with 100 μg of pcDNA3-HSP65 (lanes a and b) and from nonimmunized mice (lanes c and d) (negative control) were submitted to Southern blot after Nde I digestion. Lanes e and f correspond to wild-type plasmid digested with Nde I or undigested, respectively. The bands were detected using pcDNA3 labeled with chemiluminescent reagent. The multiple forms of plasmid DNA are indicated in the figure. The samples were loaded in a same gel and the lanes not used were removed.