TABLE 1.
Oligonucleotides
| Name/regiona | Sequence (5′→3′) |
|---|---|
| O1/traH upstream | CGAAGGGTATCAACCCCGGT |
| O2/traH upstream | TGCGGTCCCAGAAGTTAGCC |
| O3/traH upstream | CCATAGCTATTAGCCGCATC |
| O4/traH | ATGAGACTATTGGAGCAGCC |
| O4R/traH | GGCTGCTCCAATAGTCTCAT |
| O5/traI | CAACCAATCAGCCCAGGTTT |
| O5R/traI | AAACCTGGGCTGATTGGTTG |
| O6/traI | ACCATCCTACTCAGAGGGCG |
| O7/traJ upstream | CTTTACCCACTCGCTCCTGT |
| O8/traJ | AAGCTTAAAACACCCACCAG |
| O9/traK | GTAAAGCCCCGAATTTCGG |
| O10/traM | AATGCGGCATTAGCTAGTAG |
| O11/traM downstream | CCAGAGCTTAGTTGGACATT |
| lvh1/prpA | GTTTTAATCCCCCAGCAAGC |
| lvh2/prpA | AATATCCCTACTCATCCTCG |
| lvh3/lvhB3 | GGCTAGGAGGTTCTTGTG |
| lvh4/lvhB4 | ATTGGCCGAGATGTCCTT |
| lvh5/lvhB8 | CCTCTACGCATTACAACGCC |
| lvh6/lvhB9 | GTGGTGGTAAAGGGAATGCC |
| lvr1/lvrE | GGTCCAATGGGTCCAGCAGG |
| lvr2/lvrE | AGTGGCTGATTCTGGAGTGG |
| enh1/enhC | AATGCTTTGTATGCCCTCGG |
| enh2/enhC | CATATCAGCGCTTTGGCCATC |
| rtx1/rtxA | GATCCGCAAGTAGCGCTCAC |
| rtx2/rtxA | TGTAATGCTGGCATTAGGCG |
| rtx3/rtxA | CTGATGCTGCTACGGAACAC |
| rtx4/rtxA | CCGCAGTCATTACACCTGCG |
a
Each oligonucleotide was given a designation as shown in Fig. 1. Region corresponds to the gene that the oligonucleotide is located within, 5′ of (upstream) or 3′ of (downstream).