TABLE 4.
Replication of different L. pneumophila strains in mouse lungs
| Route of inoculation,a mouse strain, and experimental groupc | No. of bacteria in lungs (CFU/g)b after:
|
||
|---|---|---|---|
| 4 h | 1 day | 2 days | |
| Intranasal | |||
| C57BL/6J | |||
| AA100 | (6.2 ± 0.4) × 103 | (8.2 ± 0.2) × 103 | (1.3 ± 0.2) × 104d |
| JR32 | (5.1 ± 0.4) × 103 | (7.1 ± 0.3) × 103 | (9.5 ± 0.4) × 103d |
| Lp01 | (4.8 ± 0.4) × 103 | (5.5 ± 0.3) × 103 e | (3.4 ± 0.2) × 103e |
| A/J | |||
| AA100 | (3.9 ± 0.3) × 103 | (5.3 ± 0.5) × 103 | (1.2 ± 0.2) × 104d |
| JR32 | (3.5 ± 0.4) × 103 | (7.1 ± 0.2) × 103 | (9.8 ± 0.3) × 103d |
| Lp01 | (1.1 ± 0.3) × 103 | (3.3 ± 0.4) × 103 e | (5.1 ± 0.3) × 103d,e |
| Intratracheal | |||
| C57BL/6J | |||
| AA100 | (5.6 ± 0.2) × 105 | (6.1 ± 0.4) × 106 d | (9.2 ± 0.2) × 106d |
| JR32 | (2.3 ± 0.3) × 105 | (3.1 ± 0.2) × 105 e | (6.5 ± 0.4) × 105d,e |
| Lp01 | (4.3 ± 0.3) × 105 | (6.7 ± 0.2) × 105 e | (3.3 ± 0.4) × 105e |
| A/J | |||
| AA100 | (3.5 ± 0.4) × 105 | (7.1 ± 0.2) × 105 d | (5.3 ± 0.3) × 106d |
| JR32 | (1.3 ± 0.3) × 105 | (6.3 ± 0.4) × 105 d | (2.4 ± 0.2) × 106d,e |
| Lp01 | (2.6 ± 0.2) × 105 | (4.7 ± 0.4) × 105 e | (7.2 ± 0.3) × 105d,e |
a
An inoculum of 107 bacteria was used for all experimental groups.
b
Data represent the means ± standard deviations of duplicate platings from all mice in each group.
c
There are 12 mice in each experimental group.
d
Significantly different (P < 0.05) from the number of bacteria at 4 h.
e
Significantly different (P < 0.05) from strain AA100.